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Mapping of the immunodominant regions of the NAD-dependent formate dehydrogenase |
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| Authors: | Anna V Bogdanova Tatyana V Cherednikova Tsezi A Egorov Emil G Harutyunyan Nataleya A Kurochkina Victor S Lamzin Alexsander P Savitskiy Igor A Shumilin Vladimir O Popov |
| Institution: | Physiological Laboratory, University of Liverpool, UK. |
| Abstract: | A panel of 4 monoclonal antibodies and 7 polyclonal antisera against NAD-dependent formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101 has been obtained. The reactivity of the 37 overlapping proteolytic peptides with the monoclonal antibodies and polyclonal antisera has been studied with ELISA test. The data obtained were interpreted residing on the structural model of the formate dehydrogenase at 3 Å resolution. The immunodominant regions in the formate dehydrogenase molecule and the epitopes for the monoclonal antibodies were elucidated. |
| Keywords: | Epitope mapping Antigenic determinant Immunodominant region Formate dehydrogenase |
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