Calcium and magnesium induced changes in the relative fluidity of phosphatidylcholine liposomes |
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Authors: | R K Mishra Gauri S Singhal |
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Institution: | (1) School of Life Sciences, Jawaharlal Nehru University, 110 067 New Delhi, India |
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Abstract: | The effect of Ca2+ and Mg2+ on relative fluidity of phosphatidylcholine liposomes was studied by measuring the degree of chlorophyll fluorescence polarization.
An increase in the degree of fluorescence polarization was observed on incubation of liposomes with different concentrations
of Ca2+ or Mg2+. The results have been interpreted on the basis of increase in the size of liposomes which could be brought about by calcium
or magnesium induced fusion of small unilamellar liposomes to form larger vesicles. Fusion of liposomes has also been confirmed
by the experiments on efficiency of energy transfer from chlorophyll b to chlorophyll a, and transmission electron microscopy
of liposomes before and after incubation with Ca2+ and Mg2+. |
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Keywords: | Liposomes fluidity fluorescence polarization phosphatidylcholine |
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