Preliminary studies on the growth of Pseudomonas sp. BA2 and the production of L-aminoacylase |
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Authors: | A Bódalo J Bastida J L Gómez E Gómez M L Asanza I Alcaraz |
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Institution: | (1) Departamento de Ingeniería Química. Grupo de Ingeniería Bioquímica. Facultad de Química, Universidad de Murcia, Campus de Espinardo, 30071 Murcia, España |
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Abstract: | Summary The bacterial strain Pseudomonas sp. BA2 did not develop l-aminoacylase activity in the absence of N-acetyl-dl-alanine. The maximum growth rate and enzyme production were obtained when the acetylated amino acid was used as the sole carbon and nitrogen source. A maximum biomass of A660=1.543, after 24 h of cultivation, was obtained. The l-aminoacylase activity reached the maximum value (5.6 U ml–1 broth) in the stationary growth phase. |
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