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Alterations upstream from the Shine-Dalgarno region and their effect on bacterial gene expression |
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| Authors: | P Stanssens E Remaut W Fiers |
| Institution: | Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat 35, B-9000 Ghent Belgium Tel. 33-91-227821 |
| Abstract: | |
| Keywords: | Recombinant DNA synthetic ribosome-binding site mRNA structure fibroblast interferon β-galactosidase β-gal β-galactosidase bp base pairs IFN-β β-interferon IU international units LB Luria broth nt nucleotide(s) ONPG PA poly-acrylamide major leftward bacteriophage λ promoter RBS ribosome-binding site (region protected by the bound ribosome) SD Shine-Dalgarno sequence (sequence complementary to the 3' end of 16S RNA) SDS sodium dodecyl sulfate U unit(s) |
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