中国国内首次发现Ⅰ基因型乙型肝炎病毒基因序列

对云南省参加健康体检人员中一份乙肝表面抗原阳性标本S区基因序列测定,以了解其分子生物学特点。利用巢式PCR扩增HBV S基因片段,并对扩增产物进行序列测定,将基因序列提交到GenBank上进行BLAST搜索,同时运用Mega软件进行同源性分析,并构建基因树,并将其核苷酸和氨基酸与已报道的A~I基因型参考株的同源性进行比较。核苷酸和氨基酸比较结果证明,此标本病毒基因型为HBV I基因型。本文所发现的I基因型标本为国内首次报道。

Molecular identification of hepatitis B virus genotype I in Yunnan Province, the People's Republic of China

Molecular typing was conducted according to the reported method for one HBsAg positive carrier who had a physical examination in Yunnan Province. The S gene of this HBV sample was amplified by nested PCR and the PCR products were directly sequenced. Blast searching was done on the Genbank database and the sequence were compared with the HBV reference sequences in database. The phylogenetic tree was constructed. Homology analysis of nucleotide and smino acid were performed between the sequences from the sample and the reference sequences corresponding to HBV genotype A to I. Analysis of nucleotide and amino acid identities suggested that the sample belonged to HBV genotype I. The HBV genotype I is the first reported in China.