| 通过基因枪轰击转化获得转基因小麦植株的研究 |
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| 引用本文： | 黄粤 柯遐义. 通过基因枪轰击转化获得转基因小麦植株的研究[J]. 西北植物学报, 1997, 17(2): 142-146 |
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| 作者姓名： | 黄粤 柯遐义 |
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| 作者单位： | 中山大学生物工程研究中心 |
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| 基金项目： | “863”高科技项目资助 |
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| 摘 要： | 利用ＪＱ－７００型高速基因枪将ｐＤＭ３０２质粒ＤＮＡ上的ｂａｒ基因即ＰＡＴ酶基因导入了冬小麦品种“农大１４６”的幼胚中。经过在含有的选择培养基上筛选,得到了９块具有ｐｐｔ抗性的愈伤组织。ＰＣＲ电泳检测与ＰＣＲ－Ｓｏｕｔｈｅｒｎ发要交结果显示,外源ｂａｒ基因已转化进了由其中４块愈伤组织再生出的转基因的小麦植株中。
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| 关 键 词： | 小麦 Bar基因 基因枪 转基因植株 |
TRANSGENIC WHEAT PLANTS BY MICROPROJECTILE BOMBARDMENT OF IMMATURE WHEAT EMBRYOS |
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| Huang Yue,Ke Xiayi and Li Baojian. TRANSGENIC WHEAT PLANTS BY MICROPROJECTILE BOMBARDMENT OF IMMATURE WHEAT EMBRYOS[J]. Acta Botanica Boreali-Occidentalia Sinica, 1997, 17(2): 142-146 |
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| Authors: | Huang Yue Ke Xiayi Li Baojian |
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| Abstract: | The bar gene (phosphinothricin acetyltransferase gene) in plasmid pDM302 hadbeen transferred into the immature embryos of a winter wheat cultivar (Triticum aestivum L.) "Nong Da 146" by microprojectile bombardment with JQ-700 system.Afterselection for 2-3 months,we have obtained 9 pieces ppt (phosphinothricin)-resistant calluses.PCR electrophoresis assay and PCR-Southern hybridization showed the foreignbar gene had been stably transformed into the genomes of the transgenic wheat plantletsregenerated from 4 of the 9 ppt-resistant calluses. |
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| Keywords: | wheat bar immature embryo microprojectile bombardment transgenic plant |
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