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Substrate specificity of restriction endonuclease VspI |
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| Authors: | S Kh Degtiarev V E Repin N I Rechkunova V E Chizhikov E G Malygin |
| Abstract: | The recognition sequence and cleavage point of restriction endonuclease VspI have been determined as 5'-AT decreases TAAT. This enzyme is not isoschizomer of any known restriction endonucleases. DNA pBR322 contains a single VspI recognition sequence in position 3539. Therefore this enzyme may be used for cloning DNA in the VspI site in AmpR-gene of pBR322. |
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