| 单颗粒电子显微学的研究进展 |
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| 引用本文： | 蔡刚. 单颗粒电子显微学的研究进展[J]. 生物物理学报, 2010, 0(7) |
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| 作者姓名： | 蔡刚 |
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| 作者单位： | Department of Cell Biology;The Scripps Research Institute; |
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| 基金项目： | supported by a grant from NIH(R01GM67167) |
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| 摘 要： | 单颗粒电子显微学是一种新型的结构生物学技术和方法,一方面,其解析生物大分子复合体结构的分辨率日益提高,可以达到近原子分辨率,提供大蛋白分子或复合体的精细结构;另一方面,还可以解析生物大分子在不同功能状态下的结构及变化,对于揭示生物大分子复合体结构的作用机理具有重要作用。本文就单颗粒电子显微学的研究进展作一综述。
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| 关 键 词： | 单颗粒电子显微学 生物大分子复合体 自然生理条件 冷冻电镜 近原子分辨率 动态过程 |
State-of-The-Art of Single Particle EM |
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| CAI Gang. State-of-The-Art of Single Particle EM[J]. Acta Biophysica Sinica, 2010, 0(7) |
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| Authors: | CAI Gang |
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| Affiliation: | CAI Gang Department of Cell Biology,The Scripps Research Institute,CA 92037,USA |
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| Abstract: | Single particle EM is a powerful technique for structural characterization of macromolecular complexes in their close to native state. The EM specimen is prepared and imaged under near-physiological conditions and can provide structural information up to 0.3 nm resolution. More excitingly, single particle EM can characterize macromolecular complex in distinct functional states (including composition, conformation and interaction states) and determine structural models for transient functional states, which ... |
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| Keywords: | Single particle EM Macromolecular complex Native state Cryo-EM Near-atomic resolution Dynamic process |
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