High transfection efficiency of neural stem cells with magnetofection |
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Authors: | Sapet Cédric Laurent Nicolas de Chevigny Antoine Le Gourrierec Loïc Bertosio Elodie Zelphati Olivier Béclin Christophe |
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Affiliation: | OZ Biosciences, Marseille, France. csapet@ozbiosciences.com |
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Abstract: | Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation. |
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