Immunofluorescent staining of kinetochores in micronuclei: a new assay for the detection of aneuploidy |
| |
Authors: | F Degrassi C Tanzarella |
| |
Affiliation: | Dipartimento Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italy. |
| |
Abstract: | The immunofluorescent staining of kinetochores in micronuclei with antikinetochore antibodies was used to develop an in vitro assay for aneuploidy-inducing agents. The results show that about 80% of micronuclei induced by either colchicine or chloral hydrate contained kinetochores; only 9% of X-ray-induced micronuclei reacted positively to the antibody. These findings indicate that the in vitro micronucleus assay coupled with immunofluorescent staining of kinetochores can be a useful method for assessing the ability of chemicals to induce aneuploidy and/or chromosome aberrations. |
| |
Keywords: | |
|
|