Lysogenic induction of lambdoid phages in lexA mutants of Escherichia coli |
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Authors: | Steven G. Sedgwick Geoffrey T. Yarranton Roy W. Heath |
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Affiliation: | (1) Genetics Division, National Institute for Medical Research, Mill Hill, NW7 1AA London, Great Britain |
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Abstract: | Summary UV irradiation of lexA3 mutants of E. coli caused lysogenic induction of prophage , i21, i434 and 80. Maximal induction in lexA3 lysogens needed less UV than in lexA+ bacteria and gave 25–100% of the normal levels of infective centres induced. Assays of gene expression arising from derepression of a defective prophage showed at least 40% of the normal levels of induction by mitomycin C in lexA3 bacteria. The need for post-irradiation protein synthesis for lysogenic induction in lexA3 lysogens was reduced by increasing the basal level of recA protein with a recA+ plasmid. It is concluded that in lexAE. coli some recA protein synthesis, too small to be detected by physical means, is needed for UV induced lysogenic induction. |
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