| 海马脑片抗癫痫药物研究的离体模型 |
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| 作者姓名： | Feng WW Zhou XC Bai HB Zheng XX |
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| 作者单位： | 浙江大学生命科学与医学工程学系, |
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| 基金项目： | 浙江省心脑血管、神经系统药物评价与中药新药开发重点实验室资助项目(99106) |
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| 摘 要： | 目的：建立离体海马脑片癫痫样放电模型并用于抗癫痫药物研究。方法：在豚鼠海马脑片上灌流青霉素建立颠阗痫样放电的离体模型。并用此模型对抗癫痫药物苯巴比妥钠和苯妥英钠两种药物在不同浓度下对癫痫样放电的对抗作用进行了定量分析,结果：在海马脑片上灌流致痫药物可建立一个较好的离体组织癫痫样放电模型,苯的对抗作用进行了定量分析,结果：在海马脑片上灌流致痫药物可建立一个较好的离体组织癫痫样放电模型。苯巴比妥和苯妥英钠在一定浓度下均有显著对抗癫痫样放电的作用,且与整体实验的结果相一致。结论：本实验建立有离体脑片模型具有实验手段简单,方法灵活,易于建立药物量效关系等优点,可用于抗癫痫药物筛选和研究。
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| 关 键 词： | 海马脑片 癫痫样放大模型 青霉素 苯巴比妥钠 苯妥英钠 抗癫痫药物 |
| 文章编号： | 1000-6834 (2001)03-0302-03 |
| 修稿时间： | 1999年12月14 |
Hippocampal slice-a model in vitro for screening antiepileptic drugs |
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| Feng WW,Zhou XC,Bai HB,Zheng XX.Hippocampal slice-a model in vitro for screening antiepileptic drugs[J].Chinese Journal of Applied Physiology,2001,17(3):302-304. |
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| Authors: | Feng W W Zhou X C Bai H B Zheng X X |
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| Institution: | Department of Life Science & Biomedical Science, Zhejiang University, Hangzhou 310027, China. |
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| Abstract: | Aim: To establish a model in vitro for screening antiepleptic drugs.Methotls: Perfusing guinea pig hippocampal slice with penicillin offers a stable epileptiform discharge which was tested by two commonly clinically used antiepileptic drugs, phentoyin sodium and phenobarbital sodium at different concentration. Results:The experimental result showed that penicillin induced epileptiform discharge in the hippocampal slice and two antiepileptic drugs, phentoyin sodium and phenobarbital sodium inhibition of this discharge induced by penicillin. Conclusion: This electrophysiological model has the advantage of simplicity, flexibility and ease of quantitation and has potential value for screen antiepileptic drugs and epilepsy research. |
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| Keywords: | hippocampal slice epileptiform discharge model penicillin phenobarbital phentoyin |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
