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Microsatellite markers for Bistorta vivipara (Polygonaceae)
Authors:Vik Unni  Carlsen Tor  Eidesen Pernille Bronken  Brysting Anne Krag  Kauserud Håvard
Affiliation:Microbial Evolution Research Group, Department of Biology, University of Oslo, P.O. Box 1066 Blindern, NO-0316 Oslo, Norway.
Abstract:? Premise of the study: Using genomic shotgun 454 sequencing, 50 candidate microsatellite markers were targeted for the arctic-alpine polyploid perennial herb Bistorta vivipara to distinguish between individual genets and ramets within a population. ? Methods and Results: Out of the 50 markers, 31 were polymorphic for seven test samples. We have developed a multiplex protocol for 16 of these microsatellite markers. ? Conclusions: Our results show that the microsatellite markers provide a powerful tool for the research on genetic variation of B. vivipara.
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