Condensation of activated diguanylates on a poly(C) template |
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Authors: | R Lohrmann P K Bridson L E Orgel |
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Institution: | (1) The Salk Institute for Biological Studies, 92138 San Diego, California, USA |
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Abstract: | We have studied the metal-ion catalysis of a number of reactions of the isomers of ImpGpG on a poly(C) template. In the absence of a catalytic metal ion, oligomers at least up to (pG)20 are obtained from the ImpGpG isomers in a 1-methylimidazole buffer. The Pb2+ ion improves the yield of longer oligomers and changes substantially the distribution of linkage isomers. The Pb2+ ion greatly improves the yield of longer oligomers obtained from G and ImpGpG on a poly(C) template. The self-condensation of ImpGpG in a 2, 6-lutidine buffer is much less efficient than in a 1-methylimidazole buffer. The Zn2+ greatly increases the yield of products from the 3'-5']-linked dimer, but fails to catalyze the formation of long oligomers from the 2'-5']-linked dimer. The bonds formed in the Zn2+-catalyzed self-condensation of ImpG3pG on poly(C) are mainly 3'-5']-linked. |
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Keywords: | Imidazolides Diguanylates Poly(C) Directed condensations Linkage isomerism Prebiotic |
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