| 解离增强镧系元素荧光免疫分析灵敏度的改进 |
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| 引用本文： | 赵启仁,张福华,刘洁,林汉. 解离增强镧系元素荧光免疫分析灵敏度的改进[J]. 生物化学与生物物理进展, 1994, 21(3): 255-258 |
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| 作者姓名： | 赵启仁 张福华 刘洁 林汉 |
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| 作者单位： | 中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所 |
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| 基金项目： | 国家自然科学基金资助. |
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| 摘 要： | 为提高解离增强镧系荧光免疫分析(DELFIA)的灵敏度或信／噪比，进行了一些重要的方法学研究．观察到了对于不同的铕量，荧光响应和信／噪比都随着增强液体积而明显地变化．对于确定的铕量，存在一个最佳体积，且铕量越小，其最佳体积也越小．实验中选择最佳体积是重要的．研究了增强液制备技术，发展了微滴定板条有效的清洗和干燥方法，使本底荧光明显降低．
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| 关 键 词： | 时间分辨荧光免疫分析 解离增强镧系荧光免疫分析 灵敏度 |
| 收稿时间： | 1993-05-03 |
| 修稿时间： | 1993-08-02 |
Study on Sensitivity Improvement of Dissociation Enhanced Lanthanide Fluoroimmunoassay |
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| Zhao Qiren,Zhang Fuhu,Lu Jie and Lin Han. Study on Sensitivity Improvement of Dissociation Enhanced Lanthanide Fluoroimmunoassay[J]. Progress In Biochemistry and Biophysics, 1994, 21(3): 255-258 |
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| Authors: | Zhao Qiren Zhang Fuhu Lu Jie Lin Han |
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| Affiliation: | Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences |
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| Abstract: | Factors influencing the sensitivity, or the signal/noise ratio.of dissociation enhanced lanthaqide fluoroimmunoassay (DELFIA) have been studied. The fluorescence responses and signal/noise ratios for different europium amount were shown to be changed with the volume of enhancement solution.and there was an optimum volume at a certain europium amount.The smallest europium amount leads to the smallest optimum volume.20/00 of the net fluorescence intensity was increased by using tinfoil reflection layer. Effective washing and drying methods of microtitration strips decreased background fluorescence have been developed. |
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| Keywords: | time resolved fluoroimmunoassay dissociation enhanced lanthanide fluoroimmunoassay sensitivity |
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