黄秋葵组培快繁的研究

黄秋葵 (ＨｉｂｉｓｃｕｓｅｓｃｕｌｅｎｔｕｓＬ .)为锦葵科一年生草本植物 ,别名羊角豆、秋葵。原产非洲 ,欧美及东南亚热带地区广泛栽培 (马传先 ,1999;李正应 ,1993;翁文 ,1997;雪珍等 ,1999)。黄秋葵是一种营养保健型蔬菜 ,其花、叶、芽、果均可食用 ,种子富含油脂、蛋白质及钾、钙、铁、锌、锰等矿物质 ,晒干后既可用于提取油脂和蛋白质 ,还可作为咖啡的添加剂或代用品。花、种子和根均可入药 ,对恶疮、痛疖有疗效。黄秋葵的愈伤组织在一定条件下比较容易产生体细胞胚并再生成完整植株 ,故黄秋葵的组培快繁研究也可为胚胎学研究和人…

A Study on the Rapid Propagation of Hibiscus esculentus via Tissue Culture

The sterile seedlings of Hibiscus esculentus L.were obtained via sterile germination.The stem segments with one leaf and one axillary bud could be repeatedly subcultured on the medium"MS+1 0?mg/L IBA", and a propagation coefficient is 3-4 in each subculture. KT had a strong inhibiting effect on the germination and growth of the axillary buds of Hibiscus esculentus L., and a strong dedifferentiating effect on the stem segments. About 25% of the calli, cultured on the medium"MS+0 5-1 0?mg/L IBA" for 50-60 days,transformed into somatic embryoids, which were visible to the naked eye. Most of the somatic embryoids will develope into the intact plantlets on the same medium 30 days later.