Evaluation of capillary supercritical fluid chromatography with mass spectrometric detection for the analysis of a drug (mebeverine) in a dog plasma matrix |
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Authors: | J. David Pinkston C. J. Venkatramani Linda J. Tulich Donald J. Bowling Kenneth R. Wehmeyer |
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Affiliation: | The Procter and Gamble Company, Miami Valley Laboratories, P.O. Box 398707, Cincinnati, OH 45239-8707 USA |
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Abstract: | Supercritical fluid chromatography with mass spectrometric detection was evaluated as a technique for the analysis of drugs in biological fluids. Dog plasma was spiked with a model drug, mebeverine, and with a deuterium-labeled analog of mebeverine. The spiked plasma was prepared for analysis by solid-phase extraction on octadecylsilane cartridges. Mebeverine levels in the spiked dog plasma samples were determined by interpolation from a standard curve. Accuracy and precision of the analysis were determined within and between days. In general, accuracy was found to be 100 ± 15% for plasma samples spiked with 6 to 60 ng mebeverine/ml. The relative standard deviation for replicate sample analysis over this concentration range was between 5 and 12.5%. |
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