13C-NMR studies on griseofulvin biosynthesis and acetate metabolism in Penicillium patulum |
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| Authors: | Thomas J Simpson John SE Holker |
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| Institution: | 1. Research School of Chemistry, Australian National University, P.O. Box 4, Canberra, A.C.T. 2600 Australia;2. Robert Robinson Laboratories, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, England |
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| Abstract: | Incorporations of singly and doubly-labelled acetate-13C] into griseofulvin by a mutant strain of Penicillium patulum confirm its origin from simple folding of a single heptaketide chain. An acetate ‘starter’ effect is observed in the 13C-NMR spectra of griseofulvin enriched from acetate-13C], and analysis of the 13C—13C spin—spin couplings observed indicate a rapid metabolic turnover of added acetate. Methyl, but not carboxyl, of acetate is efficiently metabolised into the C1 pool. |
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| Keywords: | Ascomycetes biosynthesis fungal polyketide griseofulvin |
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