Swine liver methylomes of Berkshire,Duroc and Landrace breeds by MeDIPS |
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Authors: | Woo Young Bang Sam Woong Kim Seul Gi Kwon Jung Hye Hwang Tae Wan Kim Moon‐Suck Ko In‐Cheol Cho Young Kuk Joo Kwang Keun Cho Jin Young Jeong Chul Wook Kim |
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Affiliation: | 1. Swine Science and Technology Center, Gyeongnam National University of Science & Technology, , Jinju, 660‐758 Korea;2. Subtropical Animal Experiment Station, National Institute of Animal Science, RDA, , Jeju, 690‐150 Korea;3. Livestock Experiment Station, Gyeongsangnamdo Livestock Promotion Research Institute, , Sancheong, 666‐962 Korea;4. Department of Animal Resources Technology, Gyeongnam National University of Science & Technology, , Jinju, 660‐758 Korea;5. National Institute of Animal Science, RDA, , Suwon, 441‐706 Korea |
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Abstract: | Using a methyl‐DNA immunoprecipitation technique in combination with next‐generation deep sequencing, we conducted comprehensive DNA methylation profiling of liver genomes from three pig breeds: Berkshire, Duroc and Landrace. The profiles revealed that the distribution patterns of methylation signals along the genome are conserved among the three pig breeds. Specifically, many signals in coding genes were found in introns, and most signals in the repetitive elements were identified in non‐long terminal repeat (LTR) retrotransposons such as long and short interspersed repetitive elements, implying a significant association with alternative splicing and expression of retrotransposable elements respectively. Differentially methylated regions among the three pig breeds were identified in the non‐LTR retrotransposons, suggesting that they may lead to differential retrotransposable element activity. Altogether, this study provides advanced swine methylome data and valuable resources for understanding the function of DNA methylation in the evolutionary divergence of different pig breeds. |
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Keywords: | DNA methylation methyl‐DNA immunoprecipitation retrotransposon swine methylome |
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