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Silicomolybdate reduction by isolated pea chloroplasts |
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| Authors: | Kenneth E. Pallett Alan D. Dodge |
| Affiliation: | School of Biological Sciences, University of Bath, Bath, BA2 7EY, England |
| Abstract: | Conditions for the optimization of silicomolybdate reduction by isolated pea chloroplasts are described. Maximum rates of reduction are related to time of addition to the chloroplasts and the presence of an oxidizing cofactor, such as ferricyanide. Silicomolybdate or silicomolybdate plus ferricyanide reduction is only partially inhibited by a concentration of CMU which totally abolishes ferricyanide reduction. Evidence for a differing response of the two reduction sites to silicomolbydate is described. |
| Keywords: | Leguminosae pea chloroplasts silicomolybdate reduction effects of ferricyanide and an electron transport inhibitor. CMU = 3-(4′-chlorophenyl)-1, 1-dimethylurea DBMIB = dibromothymoquinone and DCMU = 3-(3′,4′-dichlorophenyl)-1, 1-dimethylurea. |
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