Structural and biochemical characterization of native and recombinant single insulin-like growth factor-binding domain protein (SIBD-1) from the Central American hunting spider Cupiennius salei (Ctenidae) |
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Authors: | Trachsel Christian Widmer Christine Kämpfer Urs Bühr Claudia Baumann Tommy Kuhn-Nentwig Lucia Schürch Stefan Schaller Johann Baumann Ulrich |
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Institution: | Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland. |
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Abstract: | Cupiennius salei single insulin-like growth factor binding domain protein (SIBD-1) is an 8.6 kDa Cys-, Pro-, and Gly-rich protein, discovered in the hemocytes of the Central American hunting spider Cupiennius salei. SIBD-1 exhibits high sequence similarity to the N-terminal domain of the insulin-like growth factor-binding protein superfamily and has been reported to play an important role in the spider's immune system. Here, the recombinant expression and the elucidation of the three-dimensional structure of recombinant SIBD-1 and the characterization of the sugar moiety at Thr2 of native SIBD-1 is described in detail. |
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Keywords: | insulin‐like growth factor‐binding protein single insulin‐binding domain Cupiennius salei glycosylation |
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