Downregulation of developmentally regulated endothelial cell locus-1 inhibits the growth of colon cancer |
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Authors: | Xiaolong Zou Haiquan Qiao Xian Jiang Xuesong Dong Hongchi Jiang Xueying Sun |
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Institution: | (1) The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical College of Harbin Medical University, Harbin, 150001, PR, China;(2) Department of Molecular Medicine & Pathology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand |
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Abstract: | Developmentally regulated endothelial cell locus-1 (Del1) is an embryonic angiogenic factor expressed in early embryonic endothelial
cells, but recently has been found to be expressed in some forms of cancers including colon and breast cancers, and melanoma,
and human cancer cell lines. Overexpression of Del1 accelerates tumor growth by enhancing vascular formation, implying Del1
may be a potential target for anti-angiogenic cancer therapy. The study aims to investigate whether downregulation of Del1
could inhibit the growth of tumors established in nude Balb/c mice by subcutaneous implantation of human LS-174T colon cancer
cells. The shRNA expression vectors targeting human Del1, and vascular endothelial growth factor (VEGF) were constructed.
Gene transfection of Del1-shRNA downregulated expression of Del1 in LS-174T cells in vivo and in vitro, but did not alter the proliferative or survival properties of cells in vitro. Gene transfection of VEGF-shRNA downregulated expression of both VEGF and Del1 in LS-174T cells in vivo and in vitro. Both Del1-shRNA and VEGF-shRNA gene therapies exhibited anti-tumor activities and they also showed a synergistic effect
in suppressing growth of colon tumors by anti-angiogenesis and anti-proliferation. Although further investigation to clarify
the mechanisms explaining the role of Del1 in tumor growth, and the interaction between VEGF and Del1, is required, the results
indicate that downregulation of Del1 presents a potent therapeutic strategy to combat colon cancer. |
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