Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars |
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Authors: | F Larronde S Krisa A Decendit C Chèze G Deffieux J M Mérillon |
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Institution: | (1) Groupe d'Etude des Substances Naturelles à Intérêt Thérapeutique, EA 491, Faculté de Pharmacie, Université de Bordeaux II, 3 place de la Victoire, F-33000 Bordeaux, France Fax: +33-5-56917988 e-mail: jean-michel.merillon@phyto.u-bordeaux2.fr, FR |
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Abstract: | Sucrose was found to modulate polyphenol accumulation in Vitis vinifera cell cultures. The production of anthocyanins increased 12-fold after addition of 0.15 m sucrose, while that of stilbenes was only slightly affected. Sucrose did not play a physical role because metabolic sugars
were required for the induction of polyphenol accumulation. Indeed, the polyols, mannitol and sorbitol, had no effect on this
accumulation. We established a model system to investigate the mechanism of sucrose regulation of polyphenol production without
inhibition of grape cell growth. After addition of sucrose to the culture medium, the major sugars accumulated in grape cells
were glucose and fructose, reaching 40% of the dry weight. The increase in the level of these hexoses closely coincided with
the increase in anthocyanin accumulation in grape cells.
Received: 18 August 1997 / Revision received: 6 November 1997 / Accepted: 5 January 1998 |
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Keywords: | Vitis vinifera Cell suspension culture Stilbenes Anthocyanins Sugars |
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