Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for bifidobacterial alpha-amylase |
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| Authors: | Moon Gi-Seong Pyun Yu-Ryang Park Myeong Soo Ji Geun Eog Kim Wang June |
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| Affiliation: | Food Safety Research Division, Korea Food Research Institute, San 46-1, Baekhyun-Dong, Bundang-Gu, Seongnam, Gyeonggi 463-746, Korea. |
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| Abstract: | A recombinant DNA, encoding the chimeric protein of the signal sequence for bifidobacterial alpha-amylase mature pediocin PA-1, was introduced into Bifidobacterium longum MG1. Biologically active pediocin PA-1 was successfully secreted from the strain and showed bactericidal activity against Listeria monocytogenes and the same molecular mass as native pediocin PA-1. |
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