Requirements for heterologous production of a complex metalloenzyme: the membrane-bound [NiFe] hydrogenase |
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| Authors: | Lenz Oliver Gleiche Andrea Strack Angelika Friedrich Bärbel |
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| Institution: | Institut für Biologie, Humboldt-Universit?t zu Berlin, Chausseestrasse 117, D-10115 Berlin, Germany. |
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| Abstract: | By taking advantage of the tightly clustered genes for the membrane-bound NiFe] hydrogenase of Ralstonia eutropha H16, broad-host-range recombinant plasmids were constructed carrying the entire membrane-bound hydrogenase (MBH) operon encompassing 21 genes. We demonstrate that the complex MBH biosynthetic apparatus is actively produced in hydrogenase-free hosts yielding fully assembled and functional MBH protein. |
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