In vitro regeneration of plantlets in Brassica alboglabra |
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Authors: | K. W. Wong C. S. Loh |
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Affiliation: | (1) Botany Department, National University of Singapore, Kent Ridge, 0511, Singapore |
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Abstract: | Different vegetative parts of Brassica alboglabra seedlings and mature plants were used as explants in culture.A high frequency (60–100%) of shoot regeneration was obtained from hypocotyl explants, nodal stem segments, internodal segments and shoot apices cultured on Murashige-Skoog basal medium. Addition of 6-benzylaminopurine and kinetin increased the average number of shoots per explant. When detached and transferred to basal medium, the shoots readily developed roots. Regenerated plantlets could be successfully transplanted in soil. |
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Keywords: | Brassica alboglabra in vitro regeneration micropropagation |
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