A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress |
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Authors: | Bin Huang Chien-Hua Chu Shu-Ling Chen Hsueh-Fen Juan Yih-Ming Chen |
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Institution: | (1) Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan;(2) Department of Biotechnology, Ming Chuan University, Taoyuan, 333, Taiwan;(3) Department of Life Science, National Taiwan University, Taipei, 106, Taiwan;(4) Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, 106, Taiwan |
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Abstract: | Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant
to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung
bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was
initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins
down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation,
ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine
synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially
recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The
regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed
herein. |
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Keywords: | Proteomics Chilling Brassinosteroid Mung bean Epicotyl |
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