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Characterization of new insertion-like sequences of Enterococcus hirae and their dissemination among clinical Enterococcus faecium isolates |
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| Authors: | Leonardo A Sechi Richard Franklin Ilaria Duprè Stefania Zanetti Giovanni Fadda Lolita Daneo-Moore |
| Institution: | Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA;Dipartimento di Scienze Biomediche, Sezione di Microbiologia Clinica e Sperimentale, Universitàdi Sassari, Viale S. Pietro 43/B, 07100 Sassari, Italy;Istituto di Microbiologia, Facoltàdi Medicina e Chirurgia 'Agostino Gemelli', UniversitàCattolica del Sacro Cuore, Rome, Italy |
| Abstract: | Sequence analysis of different fragments that hybridized with a 4.5-kb EcoRI fragment originally cloned from Enterococcus hirae ATCC 9790 showed 66% homology to IS-like sequences found in staphylococci and lactococci. We tested several enterococcal ATCC strains and found that only E. hirae ATCC 9790 and Enterococcus faecium ATCC 19434 hybridized with the IS-like sequence. Moreover, we wanted to investigate the dissemination of this new IS among E. faecium strains. We analyzed 131 clinical E. faecium isolated in Italy and the USA for the presence of the IS and we found its presence in more than 63% of the isolates. The hybridization patterns obtained vary considerably between unrelated strains and allow further classification among ribotype-grouped species. |
| Keywords: | Insertion-like sequence Enterococcus DNA fingerprinting Staphylococcus |
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