In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert |
| |
Authors: | Yang J Hu Z Guo GQ Zheng GC |
| |
Institution: | (1) Institute of Cell Biology, School of Life Science, Lanzhou University, Lanzhou, 730000, P.R, China;(2) Institute of Cell Biology, School of Life Science, Lanzhou University, Lanzhou, 730000, P.R, China |
| |
Abstract: | An effective protocol has been developed for plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona), a medicinal and agronomic shrub. Adventitious shoots were obtained from 83.2% of cotyledon explants
from 3-day seedlings cultured on Murashige and Skoog (MS) medium containing 2.0 mg l−1 thidiazuron (TDZ), with an average of 9.3 shoots per explant. Individual elongated shoots were rooted on half strength MS
medium supplemented with 2.0 mg l−1 indole-3-butyric acid (IBA), with 59.3% success. Regenerated plants with well developed shoots and roots were successfully
transferred to soil, without detectable variants. Histological observation revealed that shoots developed from cotyledon explants
via organogenesis, with little callus.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | organogenesis plant regeneration Swainsona salsula Taubert |
本文献已被 SpringerLink 等数据库收录! |
|