Synthesis and characterization of a sulfhydryl-reactive rhenium metal-ligand complex |
| |
Authors: | Dattelbaum J D Abugo O O Lakowicz J R |
| |
Institution: | University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA. |
| |
Abstract: | We describe the synthesis and spectral characterization of a rhenium metal-ligand complex. This complex reacts with sulfhydryl groups via an iodoacetamide side chain on the phenanthroline ligand and displays a high limiting anisotropy near 0.35 when excited at 442 nm. When covalently linked to human serum albumin, this complex displayed a mean decay time of about 1 micros. This decay time is appropriate for measuring rotational correlation times on the microsecond time scale as may occur for large macromolecular complexes. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|