Atrazine-resistant cauliflower obtained by somatic hybridization between Brassica oleracea and ATR-B. napus |
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Authors: | P. S. Jourdan E. D. Earle M. A. Mutschler |
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Affiliation: | (1) Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902 Ithaca, NY, USA;(2) Present address: Department of Horticulture, The Ohio State University, 2001 Fyffe Ct., 43210-1096 Columbus, OH, USA |
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Abstract: | Summary Somatic hybridization between Brassica oleracea ssp. botrytis (cauliflower, 2n=18), carrying the Ogura (R1) male-sterile cytoplasm and B. napus (2n= 38), carrying a male-fertile, atrazine-resistant (ATR) cytoplasm, yielded three hybrids (2n=56) and six cauliflower cybrids (2n=18), which were selected for resistance to the herbicide in vitro. The hybrids and cybrids were male fertile and self-compatible. They contained both chloroplasts and mitochondria from the ATR cytoplasm. We found no evidence for mtDNA recombination in any of the regenerated plants. Selfed progeny of the B. oleracea atrazine-resistant cybrids were evaluated for tolerance to the herbicide in the field. Resistant plants exposed to 0.56–4.48 kg/ha (0.5–4.0 pounds/acre) atrazine in the soil showed no damage at any herbicide level, whereas plants of a susceptible alloplasmic line were severely damaged at the lowest level of herbicide application and killed at all higher levels. These atrazine-resistant cauliflower may have potential horticultural use, especially in fields where atrazine carry over is a serious problem. |
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Keywords: | Brassica oleracea Protoplast fusion Herbicide resistance Cytoplasmic traits |
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