Synthesis of a more stable osmium ammine electron-dense DNA stain |
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Authors: | A L Olins B A Moyer S H Kim D P Allison |
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Institution: | University of Tennessee-Oak Ridge, Graduate School of Biomedical Sciences. |
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Abstract: | Specific DNA staining for electron microscopic observation is simplified by a shorter synthesis of the staining reagent. The new, more reliable reagent, osmium ammine-B, is stable for more than a year, dissolves completely in water, and does not require reoptimization of staining conditions for every batch, yet reproducibly gives strong contrast to DNA-containing structures. |
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