Role of polyamines in the cytokinin-dependent physiological processes. I. Effect of benzyladenine on polyamine levels during chloroplast differentiation in the tissue culture of Dianthus caryophyllus |
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Authors: | Jolanta Legocka Anna ?arnowska |
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Institution: | (1) Laboratory of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, al. Niepodległości 14, 61-713 Poznań |
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Abstract: | Tissue culture of Dianthus caryophyllus L. (cv. William Sim.) obligatory requiring N6-benzyladenine for greening provides a good system to study the interactions between cytokinins and polyamines. Polyamines
were analyzed as dansyl derivatives which are separated by thin layer chromatography and detected by fluorescence spectrophotometry.
Green callus growing on benzyladenine — containing medium showed decrease in the contents of free, conjugated and bound putrescine
and spermidine in comparison to chlorophyll-less callus (control callus) growing on cytokinin-free medium. The level of spermine
free, conjugated and bound forms increased about 6 %, 77 % and 28 % respectively in tissue culture growing in the presence
of cytokinin.
Spermidine was dominant polyamine bound to chromatin isolated from control callus. Chromatin isolated from green callus was
characterized by a lower level of each polyamine in comparison to chlorophyll-less callus.
Polyamines were found in plastid membrane fraction isolated from chlorophyll-less and green callus. A significant increase
the levels of polyamines (putrescine, spermidine and spermine) bound to plastid membranes in green callus (+ benzyladenine)
in comparison to chlorophyll-less callus (− benzyladenine) was observed. Additionaly, methylglyoxal-bis(guanylhydrazone) an
inhibitor of S-adenosylmethionine decarboxylase depressed the greening process.
Our results suggest that cytokinin-induced chloroplast differentiation in carnation tissue culture may be partly mediated
through the polyamines bound to thylakoid membranes. A possible role of polyamines during cytokinin-induced formation of photosynthetic
apparatus is discussed. |
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Keywords: | benzyladenine callus chromatin Dianthus caryophyllus thylakoid membranes polyamines |
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