Genetic diversity and species-specific PCR-based markers from AFLP analyses of Thai bananas |
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Authors: | Sirapope Wongniam Jamorn Somana Sasivimon Swangpol Tosak Seelanan Piyarat Chareonsap Supachitra Chadchawan Thaya Jenjittikul |
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Institution: | 1. Master of Science Programme in Plant Science, Department of Plant Science, Faculty of Science and Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand;2. Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand;3. Department of Plant Science, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand;4. Department of Botany, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand;5. Plant Genetic Conservation Project under the Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn, Ratchawithi Road, Dusit, Bangkok 10303, Thailand |
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Abstract: | A large amount of banana genetic resource has been found in Thailand which is believed to be one of the centers of its origins. To assess genetic diversity and determine genetic relationships of edible bananas in Thailand, 110 accessions of banana species and cultivars collected from villages and natural locations were investigated. UPGMA clustering of numerical data from Amplified Fragment Length Polymorphism (AFLP) patterns showed two large groups which corresponded to genome designations of Musa acuminata (AA) and Musa balbisiana (BB), the known ancestors of most edible cultivars. The AFLP data suggested that among Thai bananas, AA and AAA cultivars were closely related to M. acuminata subsp. malaccensis, while some of ‘B’ genome contained ones closely related to wild M. balbisiana in Thailand and some may have been imported. Eight species-specific PCR-based primer pairs, generated from the AFLP results clearly identify ‘A’ and ‘B’ genomes within cultivars and hybrids. The analyses were useful to readily and easily infer progenitors of these cultivars and pronounce wide genetic diversity of the bananas in Thailand. |
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Keywords: | AFLP Genetic diversity Interspecific hybrids Musa cultivars DNA fingerprinting |
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