Molecular phylogeny of the Pycnonotus sinensis and Pycnonotus taivanus in Taiwan based on sequence variations of nuclear CHD and mitochondrial cytochrome b genes |
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| Authors: | Hsueh-Wei Chang Yii-Cheng Chou Ying-Fang Su Chun-An Cheng Cheng-Te Yao Chi-Li Tsai Hsien-Chung Lee Cheng-Hao Wen Chien-Chung Cheng |
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| Affiliation: | 1. Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;2. Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;3. Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Taiwan;4. Department of Medical Technology, Chung Hwa University of Medical Technology, Tainan, Taiwan;5. Taiwan Endemic Species Research Institute, Nantao, Taiwan;6. Institute of Biodiversity, National Cheng Kung University, Tainan, Taiwan;g Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan;h Department of Life Sciences, National Sun Yet-Sen University, Kaohsiung, Taiwan |
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| Abstract: | Sequences of the partial 293-bp nuclear Z-chromosome-linked chromo-helicase binding protein (CHD-Z) and 729-bp mitochondrial cytochrome b (cyt b) genes were obtained from two species of the same family Pycnonotidae of Pycnonotus sinensis (P. sinensis, Chinese Bulbul) and Pycnonotus taivanus (P. taivanus, Taiwan Bulbul) distributed in Taiwan. A panel of 10 individuals (n = 5 for each) with unknown relationship was used to characterize single nucleotide polymorphisms (SNPs) of these genes. We identified 2 and 10 SNPs in CHD-Z and cyt b loci, respectively. Frequency of SNPs was 10 per every 1465- and 729-bp on average. Pairwise nucleotide divergences of CHD-Z and cyt b genes among 10 specimens ranged from 0 to 0.0034 and 0 to 0.0055, respectively. Phylogenetic analysis suggested that the P. taivanus group assignment based on the CHD-Z and cyt b sequences is obviously very similar to P. sinensis. |
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| Keywords: | Chinese Bulbul CHD-Z CHD-W cytochrome b SNP Taiwan Bulbul |
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