Molecular species identification of commercially important penaeid shrimp from the Gulf of Mexico using a multiplex haplotype-specific PCR assay |
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Authors: | Jaime R Alvarado Bremer James G Ditty Jennifer S Turner Brandon L Saxton |
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Institution: | 1. Department of Marine Biology, Texas A&M University, Galveston, TX 77551, USA;2. Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX 77843, USA;3. National Oceanographic and Atmospheric Administration, National Marine Fisheries Service, 4700 Avenue U, Galveston, TX 77551, USA |
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Abstract: | This study describes a multiplex PCR assay based on the 16S rRNA mitochondrial gene to identify the penaeid shrimp Farfantepenaeus aztecus, Farfantepenaeus duorarum, Farfantepenaeus brasiliensis and Litopenaeus setiferus, all native to the Gulf of Mexico, and the exotic Litopenaeus vannamei. The assay was validated using positively identified adult shrimp and confirmed by direct sequencing. Samples of postlarvae and early juveniles collected in the eastern and western Gulf of Mexico were tested yielding 119 F. aztecus, 78 F. duorarum and five L. setiferus. Reliable identification of the morphologically similar early life stages of F. aztecus and F. duorarum has important implications for management and conservation. Similarly, the ability to identify L. vannamei is relevant as early detection could help minimize the ecological impact if this species escapes to the wild. |
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Keywords: | Multiplex PCR Penaeid shrimp Species identification Farfantepenaeus aztecus F brasiliensis F duorarum Litopenaeus vannamei L setiferus Brown shrimp Pinkspot shrimp Pink shrimp Northern white shrimp Pacific white shrimp |
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