Contents of Macroelements and Growth of Sweet Cherry Rootstock in vitro |
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Authors: | Đ. Ružić M. Sarić R. Cerović Lj. Ćulafić |
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Affiliation: | (1) Plant Tissue Culture Laboratory, University of Latvia, Kandavas 2, Riga, LV-1083 Latvia |
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Abstract: | Rhododendron shoot regeneration was accomplished using either flower explants (each consisting of ovary with pedicel) of Rhododendron cvs. Nova Zembla and Irina or leaves isolated from in vitro grown Rhododendron catawbiense Michx. Multiple shoot tip clumps were obtained on Anderson's medium containing 0.5 to 1.5 mg dm−3 thidiazuron (TDZ) in combination with 12 to 15 mg dm−3 N6-[2-isopentenyl]adenine (2iP) and 1 to 3 mg dm−3 indole-3-butyric acid (IBA). After 16 weeks on the regeneration media, explants with shoot tip clumps were transferred for shoot elongation to Anderson's medium with 3 mg dm−3 2iP. Two months later, the shoots have reached 5 to 40 mm in length and were fit for subcultivation. This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | indole-3-butyric acid micropropagation N6-[2-isopentenyl]adenine thidiazuron |
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