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Fruit body formation of <Emphasis Type="Italic">Tylopilus castaneiceps</Emphasis> in pure culture
Authors:Kensuke Kikuchi  Norihisa Matsushita  Kazuo Suzuki
Institution:(1) Kazusa Research Base for Global Environments, Graduate School of Frontier Sciences, The University of Tokyo, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan;(2) Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;(3) Forestry and Forest Products Research Institute, Ibaraki, Japan
Abstract:Fruit bodies of Tylopilus castaneiceps were formed on Ohta medium in pure culture. The mycorrhizal status of T. castaneiceps was confirmed by DNA analysis of the internal transcribed spacer region of mycorrhizae collected beneath its fruit bodies. However, fruiting ability was lost within 1 year of isolation, as has been reported for most of the other ectomycorrhizal species that produce fruit bodies in pure culture without host plants.
Keywords:DNA analysis  Ectomycorrhizal fungi  Fruit body formation  Pure culture            Tylopilus castaneiceps
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