MMP-14 mediated MMP-9 expression is involved in TGF-beta1-induced keratinocyte migration |
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Authors: | Seomun Young Kim Jong-Tak Joo Choun-Ki |
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Institution: | Laboratory of Ophthalmology and Visual Science, Korean Eye Tissue and Gene Bank Related to Blindness, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. |
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Abstract: | The importance of expression of matrix metalloproteinase (MMP) in keratinocyte migration is well established, but its role remains unclear. Here we investigated the function of MMP-14 in TGF-beta1-induced keratinocyte migration. TGF-beta1 stimulated cell migration and the expression of MMP-2, -9 in HaCaT human keratinocyte cells. When we lowered MMP-14 mRNA with siRNA, cell migration, and MMP-9 expression decreased. Furthermore, the MMP-14 siRNA also reduced activation of JNK in response to TGF-beta1, and a JNK-specific inhibitor decreased both cell migration and MMP-9 expression. Taken together, these results suggest that TGF-beta1-induced HaCaT cell migration is mediated by MMP-14, which regulates MMP-9 expression via JNK signaling. |
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Keywords: | cell migration MMP TGF‐beta1 JNK |
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