Different feeding strategy for the production of biosurfactant from Pseudomonas aeruginosa USM AR2 in modified bioreactor |
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Authors: | M S Salwa M N Nur Asshifa A A Amirul Ahmad R M Yahya |
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Institution: | (1) Department of Molecular Biology & Biotechnology, Tezpur University, Napaam, Tezpur, Assam, 784028, India |
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Abstract: | A locally-isolated Pseudomonas aeruginosa USM AR2 possessing the ability to produce glycolipid-type biosurfactant (rhamnolipid) was used in this research to explore
fermentation technology for rhamnolipid production. Rhamnolipid concentration in 2.5 L fed-batch fermentation was improved
from 0.173 to 8.06 g/L by manipulating the feeding strategy and cultivation protocol. The culture was fed with petroleum diesel
and complex medium. The highest rhamnolipid concentration was achieved when the culture was initially fed with both petroleum
diesel and complex medium, followed by feeding of petroleum diesel only at the end of the stationary phase. Severe foaming
problem was resolved by modifying and integrating a foam recycler to the bioreactor. This successfully extended the cultivation
period and increased the yield of final rhamnolipid. No antifoam agent was added as this modified bioreactor allowed cultivation
to proceed even under foam generation. The viscosity measurement, surface tension activity test, and drop-collapse test were
performed as an indirect measure of rhamnolipid presence. |
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