Transferrin variants in sheep: separation and characterization by polyacrylamide gel electrophoresis and isoelectric focusing* |
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Authors: | G. ERHARDT |
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Affiliation: | Institute for Animal Breeding, University of Giessen, West Germany |
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Abstract: | Summary. Isoelectric focusing with carrier ampholytes in ultrathin polyacrylamide gels and polyacrylamide gel electrophoresis in a discontinuous buffer system were used for the separation of sheep transferrin variants. For identification of the different iron-binding sites of transferrin a stepwise urea gradient, different degrees of iron saturation and double one-dimensional electrophoresis were used. Isoelectric focusing results in an increased resolution of the Fe0-transferrin, Fe1-transferrin and Fe2-transferrin region. At the level of Fe0-transferrin and Fe1-transferrin the variants I, A, G, B, C, D, M, E, Q, P can be identified. The method is especially suitable for genetic studies. For screening purposes up to 108 samples can be separated within one run in an ultrathin gel. |
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Keywords: | sheep genetic polymorphism transferrin polyacrylamide gel electrophoresis isoelectric focusing |
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