Oxygen utilization by Lactobacillus plantarum |
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| Authors: | F. Götz E. F. Elstner B. Sedewitz E. Lengfelder |
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| Affiliation: | (1) Institut für Botanik und Mikrobiologie der Technischen Universität München, Arcisstr. 2, D-8000 München 2, Germany;(2) Strahlenbiologisches Institut der Universität München, Bavariaring 19, D-8000 München 2, Germany |
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| Abstract: | Cell-free extracts of Lactobacillus plantarum contain non-proteinaceous compounds which mimic superoxide dismutase activity. Using the test system in which O2–is generated by xanthine oxidase, superoxide dismutase activity is found in cell-free extracts, where proteins are removed by precipitation. This activity is strongly decreased after dialysis of cell-free extracts. Superoxide dismutase activity was also investigated by means of pulse radiolysis. Cell-free extracts of Escherichia coli were also investigated as a comparison, which were known to contain superoxide dismutase. With cell-free extracts of both L. plantarum and E. coli the decay of O2–was markedly increased. However, the type of reaction of the O2–decay was of first order in the presence of E. coli extracts due to superoxide dismutase(s), and of second order in the presence of L. plantarum extracts, indicating that O2–elimination is not an enzymic reaction. Mn2+ phosphate(s) might be responsible for the observed elimination of O2–. The production of O2–is not detectable during NADH-, lactate- or pyruvate oxidase reactions in L. plantarum extracts. |
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| Keywords: | Lactobacillus plantarum Superoxide dismutase Superoxide anion Pulse radiolysis |
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