A combined PRINS-FISH technique for simultaneous localisation of DNA sequences on plant chromosomes |
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| Authors: | M Kubaláková M Nouzová M Doleželová J Macas J Doležel |
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| Institution: | (1) De Montfort University Norman Borlaug Centre for Plant Science, Academy of Sciences of the Czech Republic, Sokolovská 6, CZ-77200 Olomouc, Czech Republic;(2) Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, CZ-77200 Olomouc, Czech Republic;(3) Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ-37005 České Budějovice, Czech Republic |
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| Abstract: | A novel approach for simultaneous localization of two DNA sequences on plant chromosomes is described. The approach is based
on a combined use of primed in situ DNA labelling (PRINS) with fluorescent in situ hybridization (FISH). Traditionally, this
has been done using FISH with two probes labelled by two different marker molecules. Compared to this method, the combined
PRINS-FISH procedure is faster. Furthermore, because one of the DNA sequences is localized by PRINS with specific primers,
only one labelled probe is needed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | FokI repetitive sequence physical mapping 45S rRNA genes |
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