Extracellular polysaccharides of modified strains of Erwinia spp. |
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| Authors: | B Y Yang J M Brand J S Gray R Montgomery |
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| Affiliation: | Department of Biochemistry, College of Medicine, University of Iowa, 52242, Iowa City, IA, USA. |
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| Abstract: | The structure of the extracellular polysaccharide (EPS) produced by Erwinia chrysanthemi strain A2148 has been determined using low pressure size-exclusion and anion-exchange chromatographies, high pH anion-exchange chromatography, glycosyl-linkage analysis, and 1D 1H NMR spectroscopy. The polysaccharide is structurally similar, if not identical, to the EPS produced by E. chrysanthemi strain A350. A streptomycin-resistant strain of E. chrysanthemi Ech6 (Ech6S(+)) has been generated and has an elevated production of EPS, as does a streptomycin-resistant strain (Ech9Sm6) of E. chrysanthemi Ech9. These modified E. chrysanthemi spp. have been ribotyped and found to be closely related to their parent strains. |
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