Urine/Plasma Neutrophil Gelatinase Associated Lipocalin Ratio Is a Sensitive and Specific Marker of Subclinical Acute Kidney Injury in Mice |
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| Authors: | Tamás Kaucsár Mária Godó Csaba Révész Miklós Kovács Attila Mócsai Norbert Kiss Mihály Albert Tibor Krenács Gábor Szénási Péter Hamar |
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| Affiliation: | 1. Institute of Pathophysiology, Semmelweis University, Budapest, Hungary;2. MTA-SE “Lendület” Inflammation Physiology Research Group, Department of Physiology, Semmelweis University, Budapest, Hungary;3. CEVA Phylaxia Ltd., Budapest, Hungary;4. MTA-SE Tumor Progression Research Group, 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary;Emory University, UNITED STATES |
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| Abstract: | BackgroundDetection of acute kidney injury (AKI) is still a challenge if conventional markers of kidney function are within reference range. We studied the sensitivity and specificity of NGAL as an AKI marker at different degrees of renal ischemia.MethodsMale C57BL/6J mice were subjected to 10-, 20- or 30-min unilateral renal ischemia, to control operation or no operation, and AKI was evaluated 1 day later by histology, immunohistochemistry, BUN, creatinine, NGAL (plasma and urine) and renal NGAL mRNA expression.ResultsA short (10-min) ischemia did not alter BUN or kidney histology, but elevated plasma and urinary NGAL level and renal NGAL mRNA expression although to a much smaller extent than longer ischemia. Surprisingly, control operation elevated plasma NGAL and renal NGAL mRNA expression to a similar extent as 10-min ischemia. Further, the ratio of urine to plasma NGAL was the best parameter to differentiate a 10-min ischemic injury from control operation, while it was similar in the non and control-operated groups.ConclusionsThese results suggest that urinary NGAL excretion and especially ratio of urine to plasma NGAL are sensitive and specific markers of subclinical acute kidney injury in mice. |
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