盐胁迫下宁夏枸杞苯丙烷代谢相关基因差异表达分析北大核心CSCD

为探究盐胁迫条件下宁夏枸杞苯丙烷代谢相关基因差异表达规律,以不同浓度NaCl(0,100,200,300 mmol/L)处理的水培宁夏枸杞幼苗为研究材料,利用高通量测序技术和qRT-PCR对盐胁迫下宁夏枸杞苯丙烷代谢相关基因差异表达进行分析,同时对该途径中关键酶活性及产物含量进行测定。结果表明,(1)宁夏枸杞在不同浓度NaCl处理下共有58个苯丙烷代谢相关基因差异表达,且随着盐胁迫程度的增加大部分基因表达水平上调或不变;(2)随着NaCl浓度的增加,宁夏枸杞叶片抗氧化酶SOD、POD、CAT的活性均下降,而酚类物质、类黄酮和木质素的含量在100 mmol/L NaCl处理下均显著积累。研究发现,宁夏枸杞可能通过调控苯丙烷代谢相关基因上调表达,增加酚类物质、类黄酮和木质素的合成,来清除过多活性氧和提升细胞壁强度以适应盐胁迫;宁夏枸杞可耐受的NaCl浓度在100~200 mmol/L之间。

Regulation of Phenylpropane Metabolism Genes Differential Expression on Adaptation of Lycium barbarum L. to Salt Stress

To explore the mechanism of phenylpropane metabolism in adapting to salt stress of L. barbarum. In this study, L. barbarum treated with different concentrations of NaCl (0, 100, 200, 300 mmol/L NaCl) was used as the research object. High-throughput sequencing technology and qRT-PCR were used to detect and analyze the differential expression of genes related to phenylpropane metabolism of L. barbarum under salt stress. Meanwhile, the activities of key enzymes and the contents of related substances were determined. The results showed as follows: (1) A total of 58 genes related to phenylpropane metabolism were differentially expressed under different concentrations of NaCl, and most of the genes were up-regulated or unchanged with the increase of salt stress. (2) With the increase of NaCl concentration, the activities of antioxidant enzymes SOD, POD and CAT in the leaves of L. barbarum decreased, while the contents of phenols, flavonoids and lignin were significantly accumulated under 100 mmol/L NaCl treatment. The results showed that L. barbarum could regulate the up-regulated expression of genes related to phenylpropane metabolism, increase the synthesis of phenols, flavonoids and lignin, and adapt to salt stress by clearing excessive ROS and improving cell wall strength. The concentration of NaCl that L. barbarum can tolerate is between 100 and 200 mmol/L. The results of this study provide theoretical basis for the cultivation and production of L. barbarum and the further analysis of the salt tolerance mechanism of L. barbarum.