Evaluating the Impact of Breastfeeding on Rotavirus Antigenemia and Disease Severity in Indian Children |
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Authors: | Sushmita Das Ganesh Chandra Sahoo Pradeep Das Utpal Kant Singh Anil Kumar Jaiswal Prachi Singh Ranjeet Kumar Rishikesh Kumar |
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Affiliation: | 1. Department of Microbiology, All-India Institute of Medical Sciences (AIIMS), Patna, India;2. Virology Unit, Rajendra Memorial Research Institute of Medical Sciences (ICMR), Patna, India;3. Department of Paediatrics, Nalanda Medical College and Hospital, Patna, India;University of North Carolina School of Medicine, UNITED STATES |
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Abstract: | ObjectivesTo evaluate the contribution of breastfeeding to Rotavirus (RV)-induced antigenemia and/or RNAemia and disease severity in Indian children (<2 yrs age).MethodsPaired stool and serum samples were collected from (a) hospitalized infants with diarrhea (n = 145) and (b) healthy control infants without diarrhea (n = 28). Stool RV-antigen was screened in both groups by commercial rapid-test and enzyme immunoassay. The disease severity was scored and real-time-PCR was used for viral-load estimation. Serum was evaluated for RV-antigenemia by EIA and RV-RNAemia by RT-PCR. Data was stratified by age-group and breastfeeding status and compared.ResultsPresence of RV-antigenemia and RV-RNAemia was positively related with presence of RV in stool. Disease severity and stool viral-load was significantly associated with RV-antigenemia[(r = 0.74; CI:0.66 to 0.84; P<0.0001,R2 = 0.59) and (r = -0.55; CI:-0.68 to -0.39; P<0.0001,R2 = 0.31) respectively], but not with RV-RNAemia. There was significant reduction in RV-antigenemiarate in the breast-fed group compared to non-breastfed infants, especially in 0–6 month age group (P<0.001). Non-breastfed infants were at risk for RV-antigenemia with severe disease manifestations in form of high Vesikari scores correlating with high fever, more vomiting episodes and dehydration.ConclusionRV-antigenemia was common in nonbreastfed children with severe RV-diarrhea and correlated with stool RV-load and disease severity. |
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