Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation |
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| Authors: | Peter T Hallock Sherry Chin Steven Blais Thomas A Neubert David J Glass |
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| Institution: | aNovartis Institutes for Biomedical Research, Cambridge, Massachusetts, USA;bSkirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York, USA |
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| Abstract: | Crk and CrkL are noncatalytic adaptor proteins necessary for the formation of neuromuscular synapses which function downstream of muscle-specific kinase (MuSK), a receptor tyrosine kinase expressed in skeletal muscle, and the MuSK binding protein Dok-7. How Crk/CrkL regulate neuromuscular endplate formation is not known. To better understand the roles of Crk/CrkL, we identified CrkL binding proteins using mass spectrometry and have identified Sorbs1 and Sorbs2 as two functionally redundant proteins that associate with the initiating MuSK/Dok-7/Crk/CrkL complex, regulate acetylcholine receptor (AChR) clustering in vitro, and are localized at synapses in vivo. |
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