Glutathione reductase plays an anti-apoptotic role against oxidative stress in human hepatoma cells |
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| Authors: | Su-Jung Kim Hyun-Joo Jung Dong-Hoon Hyun Eun-Hee Park Young-Myeong Kim Chang-Jin Lim |
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| Institution: | 1. Division of Life Sciences and Research Institute of Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea;2. College of Pharmacy, Sookmyung Women’s University, Seoul 140-742, Republic of Korea;3. Division of Life Science, Ewha Womans University, Seoul 120-750, Republic of Korea;4. Vascular System Research Center and Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea |
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| Abstract: | The cellular roles of glutathione reductase (GR) in the reactive oxygen species (ROS)-induced apoptosis were studied using the HepG2 cells transfected with GR. The overexpression of GR caused a marked enhancement in reduced and oxidized glutathione (GSH/GSSG) ratio, and significantly decreased ROS levels in the stable transfectants. Hydrogen peroxide (H2O2), under the optimal condition for apoptosis, significantly decreased cellular viability and total GSH content, and rather increased ROS level, apoptotic percentage and caspase-3 activity in the mock-transfected cells. However, hydrogen peroxide could not largely generate these apoptotic changes in cellular viability, ROS level, apoptotic percentage, caspase-3 activity and total GSH content in the cells overexpressing GR. Taken together, GR may play a protective role against oxidative stress. |
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| Keywords: | Glutathione reductase Glutathione Apoptosis Hepatoma cells Reactive oxygen species Hydrogen peroxide HepG2 |
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