Differential behaviour of Pseudomonas sp. 42A2 LipC,a lipase showing greater versatility than its counterpart LipA |
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Authors: | Cristina Bofill Núria Prim Maria Mormeneo Angeles Manresa FI Javier Pastor Pilar Diaz |
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Institution: | 1. Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 645, 08028-Barcelona, Spain;2. Microbiology Unit, Faculty of Pharmacy, University of Valencia, Av. Vicente Andrés Estelles s/n, 46100-Burjassot, Spain;3. Laboratory of Microbiology, Faculty of Pharmacy, University of Barcelona, Joan XXIII s/n, 08028-Barcelona, Spain |
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Abstract: | Growth of Pseudomonas sp. 42A2 on oleic acid releases polymerized hydroxy-fatty acids as a result of several enzymatic conversions that could involve one or more lipases. To test this hypothesis, the lipolytic system of strain Pseudomonas sp. 42A2 was analyzed, revealing the presence of at least an intracellular carboxylesterase and a secreted lipase. Consensus primers derived from a conserved region of bacterial lipase subfamilies I.1 and I.2 allowed isolation of two secreted lipase genes, lipA and lipC, highly homologous to those of Pseudomonas aeruginosa PAO1. Homologous cloning of the isolated lipA and lipC genes was performed in Pseudomonas sp. 42A2 for LipA and LipC over-expression. The overproduced lipases were further purified and characterized, both showing preference for medium fatty acid chain-length substrates. However, significant differences could be detected between LipA and LipC in terms of enzyme kinetics and behaviour pattern. Accordingly, LipA showed maximum activity at moderate temperatures, and displayed a typical Michaelis–Menten kinetics. On the contrary, LipC was more active at low temperatures and displayed partial interfacial activation, showing a shift in substrate specificity when assayed at different temperatures, and displaying increased activity in the presence of certain heavy metal ions. The versatile properties shown by LipC suggest that this lipase could be expressed in response to variable environmental conditions. |
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Keywords: | Lipase Carboxylesterase Pseudomonas |
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